Tehran University of Medical Sciences (TUMS) Academic Journal of Surgery 2423-3218 8 1 2025 04 25 29 33 abhishek sharma senior consultant plastic surgeon aiwh burhanpur m.p 2025 01 24 Scars are abnormal changes in skin tissue caused by injuries, infections, and various factors. They commonly result from fibroblasts in affected skin synthesizing collagen, leading to irregular growth and excessive collagen accumulation within the extracellular matrix. This study aims to investigate the effects of surgical and laser methods on scar formation mechanisms and lymphatic drainage, as well as their outcomes. The objective is to assess skin scars created by laser and surgical incisions and their impact on pathophysiology and lymphatic flow in humans. A total of 20 patients with skin lesions were included in this study. The migration of methylene blue dye through the lymphatic vessels of the lower limbs was evaluated, with the administered dose limited to under 2 mg/kg. Transverse incisions were then performed distally using either a surgical blade or laser. The wounds were allowed to heal by secondary intention. The results indicated that laser-treated wounds did not heal completely, retaining a noticeable area of granulation tissue along with hair loss. In contrast, wounds created through surgical incisions healed entirely. Additionally, significantly lower dye migration levels were observed in the limbs after laser treatment compared to surgical incisions (p = 0.007). The findings suggest that scar size may be influenced by the type of incision used. However, further research is required to validate these results. https://ajs.tums.ac.ir/index.php/ajs/article/view/298