Autologous Fat Grafting Enhanced with Stromal Vascular Fraction (SVF) for Soft Tissue Reconstruction
Abstract
Autologous fat grafting continues to face a persistent challenge: retention rates that fluctuate unpredictably
between 20% and 80%. The stromal vascular fraction (SVF)—a heterogeneous cell population containing
adipose-derived stem cells and other regenerative cell types—has emerged as a promising adjunct. By
romoting angiogenesis and supporting tissue regeneration, SVF enhances graft survival [1,2].
This review examines current evidence on the mechanisms of SVF-enhanced fat grafting, the techniques used
for its isolation, and the clinical contexts in which it has been successfully applied [3]. Literature published
between 2015 and 2025 was analyzed, focusing on studies that investigated SVF-enriched fat grafting in
reconstructive surgery [4]. Evidence indicates that enrichment with SVF im proves graft retention, with rates
rising to 65–80% compared to 40–65% achieved with conventional methods. Enhanced neovascularization has
also been consistently reported [5,6]. Clinically, surgeons are applying SVF-assisted grafting in breastreconstruction, rehabilitation of radiation-damaged tissue, chronic wound management, and facial rejuvenation [7,8]. Both enzymatic digestion and mechanical separation techniques have proven effective for isolating SVF, offering flexibility depending on available resources and regulatory considerations [9,10]. Overall, SVF-enhanced fat grafting represents a significant advance in regenerative surgery. Multiple studies confirm its safety and therapeutic value, underscoring its potential to improve outcomes across a range of reconstructive and aesthetic applications [11,12].
1. Coleman SR. Structural fat grafting. Plast Reconstr Surg. 2006;118(Suppl):108S-120S. https://doi.org/10.1097/01.prs.0000234610.81672.e7
2. Gause TM, Rubin JP. Adipose stem cells and fat grafting. Clin Plast Surg. 2014;41(2):345-352.
3. Debuc B, Gendron N, Cras A. Improving fat grafting through stem cell-assisted lipotransfer. Stem Cell Rev Rep. 2023;19(6):1726-1754. https://doi.org/10.1007/s12015-023-10568-4
4. Liu M, Shang Y, Liu N. Strategies to improve AFT retention. Aesthetic Plast Surg. 2023;47(2):808-824. https://doi.org/10.1007/s00266-022-03088-y
5. Chiu CH, Hsu CC, Lin YY. Cell-assisted lipotransfer in breast augmentation. Plast Reconstr Surg Glob Open. 2019;7(10):e2400.
6. Limido KE, Ma X. Long-term quantitative analysis of fat graft retention. J Plast Reconstr Aesthet Surg. 2023;78:44-52.
7. Cai Y, Zhang F, Feng J. Long-term follow-up of SVF gel in chronic wounds. Stem Cell Res Ther. 2024;15(1):85.
8. Gentile P, Garcovich S. Systematic review of ADSCs and SVF for wound healing. Stem Cells Int. 2021;2021:6693709.
9. Uguten UO, Veldhuizen J. Mechanical and enzymatic SVF isolation. Plast Reconstr Surg Glob Open. 2024;12(1):e5534.
10. Solodeev I, Marrazzo P. Comparison of enzymatic and mechanical methods. Aesthetic Plast Surg. 2023;47(3):1045-1054.
11. Kølle SF, Fischer-Nielsen A. Enrichment of fat grafts with ADSCs. Lancet. 2013;382(9898):1113-1120. https://doi.org/10.1016/S0140-6736(13)61410-5
12. Yoshimura K, Sato K. Cell-assisted lipotransfer for breast augmentation. Aesthetic Plast Surg. 2008;32(1):48-55. https://doi.org/10.1007/s00266-007-9019-4
13. Coleman SR. Facial recontouring with lipostructure. Clin Plast Surg. 1997;24(2):347-367. https://doi.org/10.1016/S0094-1298(20)31069-5
14. Simonacci F, Bertozzi N. Autologous fat grafting procedures. Ann Med Surg. 2017;20:49-60. https://doi.org/10.1016/j.amsu.2017.06.059
15. Eto H, Kato H. Fate of adipocytes after nonvascularized fat grafting. Plast Reconstr Surg. 2012;129(5):1081-1092. https://doi.org/10.1097/PRS.0b013e31824a2b19
16. Kato H, Mineda K. Degeneration and regeneration after fat grafting. Plast Reconstr Surg. 2014;133(3):303e-313e. https://doi.org/10.1097/PRS.0000000000000066
17. Bourin P, Bunnell BA. Stromal cells from adipose SVF. Cytotherapy. 2013;15(6):641-648. https://doi.org/10.1016/j.jcyt.2013.02.006
18. Yoshimura K, Shigeura T. Characterization of liposuction aspirate cells. J Cell Physiol. 2006;208(1):64-76. https://doi.org/10.1002/jcp.20636
19. Zuk PA, Zhu M. Multilineage cells from human adipose tissue. Tissue Eng. 2001;7(2):211-228. https://doi.org/10.1089/107632701300062859
20. Gimble JM, Katz AJ. Adipose-derived stem cells for regenerative medicine. Circ Res. 2007;100(9):1249-1260. https://doi.org/10.1161/01.RES.0000265074.83288.09
21. Matsumoto D, Sato K. Cell-assisted lipotransfer. Tissue Eng. 2006;12(12):3375-3382. https://doi.org/10.1089/ten.2006.12.3375
22. Matsumoto D, Gonda K. CAL-supportive use of ADSCs. J Cell Physiol. 2007;212(3):702-709.
23. Zielins ER, Luan A. Therapeutic applications of adipose stromal cells. Discov Med. 2015;19(105):245-253.
24. Bora P, Majumdar AS. Adipose SVF in regenerative medicine. Stem Cell Res Ther. 2017;8(1):145. https://doi.org/10.1186/s13287-017-0598-y
25. Zuk PA. The adipose-derived stem cell. Mol Biol Cell. 2010;21(11):1783-1787. https://doi.org/10.1091/mbc.e09-07-0589
26. Gimble JM, Bunnell BA. Adipose-derived stromal/stem cells primer. Organogenesis. 2013;9(1):3-10. https://doi.org/10.4161/org.24279
27. Dominici M, Le Blanc K. Minimal criteria for MSCs. Cytotherapy. 2006;8(4):315-317. https://doi.org/10.1080/14653240600855905
28. Mitchell JB, McIntosh K. Immunophenotype of adipose-derived cells. Stem Cells. 2006;24(2):376-385. https://doi.org/10.1634/stemcells.2005-0234
29. Miranville A, Heeschen C. Improvement of neovascularization by ADSCs. Circulation. 2004;110(3):349-355. https://doi.org/10.1161/01.CIR.0000135466.16823.D0
30. Planat-Benard V, Silvestre JS. Plasticity toward endothelial cells. Circulation. 2004;109(5):656-663. https://doi.org/10.1161/01.CIR.0000114522.38265.61
31. Corselli M, Chen CW. Perivascular ancestors of stem cells. Arterioscler Thromb Vasc Biol. 2010;30(6):1104-1109. https://doi.org/10.1161/ATVBAHA.109.191643
32. Tang W, Zeve D. White fat progenitor cells in vasculature. Science. 2008;322(5901):583-586. https://doi.org/10.1126/science.1156232
33. Spencer M, Yao-Borengasser A. Adipose tissue macrophages. Am J Physiol Endocrinol Metab. 2010;299(6):E1016-E1027. https://doi.org/10.1152/ajpendo.00329.2010
34. Shaul ME, Bennett G. M2-like remodeling phenotypes. Diabetes. 2010;59(5):1171-1181. https://doi.org/10.2337/db09-1402
35. Rehman J, Traktuev D. Secretion of angiogenic factors by adipose stromal cells. Circulation. 2004;109(10):1292-1298. https://doi.org/10.1161/01.CIR.0000121425.42966.F1
36. Kim WS, Park BS. Wound healing effect of ADSCs. J Dermatol Sci. 2007;48(1):15-24. https://doi.org/10.1016/j.jdermsci.2007.05.018
37. Cao Y, Sun Z. ADSCs differentiate into endothelial cells. Biochem Biophys Res Commun. 2005;332(2):370-379. https://doi.org/10.1016/j.bbrc.2005.04.135
38. Nakagami H, Maeda K. Autologous cell therapy in ischemic limb. Arterioscler Thromb Vasc Biol. 2005;25(12):2542-2547. https://doi.org/10.1161/01.ATV.0000190701.92007.6d
39. Rigotti G, Marchi A. Clinical treatment of radiotherapy damage. Plast Reconstr Surg. 2007;119(5):1409-1422. https://doi.org/10.1097/01.prs.0000256047.47909.71
40. Suga H, Eto H. Adipose tissue remodeling under ischemia. Plast Reconstr Surg. 2010;126(6):1911-1923. https://doi.org/10.1097/PRS.0b013e3181f4468b
41. Puissant B, Barreau C. Immunomodulatory effect of adipose stem cells. Br J Haematol. 2005;129(1):118-129. https://doi.org/10.1111/j.1365-2141.2005.05409.x
42. Yanez R, Lamana ML. ADSCs have immunosuppressive properties. Stem Cells. 2006;24(11):2582-2591. https://doi.org/10.1634/stemcells.2006-0228
43. Casteilla L, Planat-Bénard V. Adipose stromal cells in trials. World J Stem Cells. 2011;3(4):25-33. https://doi.org/10.4252/wjsc.v3.i4.25
44. Kilroy GE, Foster SJ. Cytokine profile of ADSCs. J Cell Physiol. 2007;212(3):702-709. https://doi.org/10.1002/jcp.21068
45. Tonnard P, Verpaele A. Nanofat grafting research and applications. Plast Reconstr Surg. 2013;132(4):1017-1026. https://doi.org/10.1097/PRS.0b013e31829fe1b0
46. Jiang A, Li M. Improvement via SVF gel grafting. Stem Cell Res Ther. 2020;11(1):162. https://doi.org/10.1186/s13287-020-01811-7
47. Cohen SR, Hewett S. Regenerative cells for facial surgery. Aesthet Surg J. 2017;37(Suppl 3):S16-S32. https://doi.org/10.1093/asj/sjx078
48. Flynn L, Prestwich GD. Adipose tissue engineering with scaffolds. Biomaterials. 2008;29(3):329-338. https://doi.org/10.1016/j.biomaterials.2007.12.028
49. Rodbell M. Metabolism of isolated fat cells. J Biol Chem. 1964;239:375-380. https://doi.org/10.1016/S0021-9258(18)51687-2
50. Bunnell BA, Flaat M. ADSC isolation and expansion. Methods. 2008;45(2):115-120. https://doi.org/10.1016/j.ymeth.2008.03.006
51. US FDA. Regulatory considerations for human cells and tissues. Guidance. 2017.
52. Raposio E, Ciliberti R. Clinical use of ADSCs: legislative issues. Ann Med Surg. 2017;24:61-64. https://doi.org/10.1016/j.amsu.2017.11.002
53. Aronowitz JA, Lockhart RA. Mechanical versus enzymatic isolation. Springerplus. 2015;4:713. https://doi.org/10.1186/s40064-015-1509-2
54. Tonnard P, Verpaele A. Nanofat grafting. Plast Reconstr Surg. 2013;132(4):1017-1026. https://doi.org/10.1097/PRS.0b013e31829fe1b0
55. Solodeev I, Marrazzo P. Comparison of isolation methods. Aesthetic Plast Surg. 2023;47(3):1045-1054.
56. Uguten UO, Veldhuizen J. Systematic review of yield and viability. Plast Reconstr Surg Glob Open. 2024;12(1):e5534.
57. Aronowitz JA, Ellenhorn JD. SVF isolation head-to-head comparison. Plast Reconstr Surg. 2013;132(4):932e-939e. https://doi.org/10.1097/PRS.0b013e3182a80652
58. Yoshimura K, Asano Y. Progenitor-enriched transplantation. Breast J. 2010;16(2):169-175. https://doi.org/10.1111/j.1524-4741.2009.00873.x
59. Khouri RK, Rigotti G. Megavolume autologous fat transfer. Plast Reconstr Surg. 2014;133(3):550-557. https://doi.org/10.1097/01.prs.0000438044.06387.2a
60. Chiu CH, Hsu CC. CAL in breast augmentation. Plast Reconstr Surg Glob Open. 2019;7(10):e2400.
61. Largo RD, Tchang LA. Efficacy and safety of fat grafting. Breast. 2014;23(1):28-35.
62. Calabrese C, Garlaschi A. CAL oncological safety study. Plast Reconstr Surg. 2018;142(1):31-36.
63. Wang Y, Zhang J. Fat grafting after breast cancer surgery meta-analysis. Aesthetic Plast Surg. 2023;47(2):451-462. https://doi.org/10.1007/s00266-023-03331-0
64. Petit JY, Botteri E. Locoregional recurrence after fat grafting. Plast Reconstr Surg. 2012;129(5):1173-1175.
65. Heine N, Sorg H. Fat grafting for breast reconstruction in radiated patients. J Plast Reconstr Aesthet Surg. 2023;76:234-242.
66. Salgarello M, Visconti G. Fat grafting before tissue expander in radiated patients. Plast Reconstr Surg. 2020;145(3):619e-628e.
67. Phothong W, Zenn MR. Fat grafting for radiation ulcer. Plast Reconstr Surg Glob Open. 2017;5(10):e1523.
68. Chen L, Li C. Autologous fat grafting after breast radiotherapy. Aesthetic Plast Surg. 2021;45(4):1565-1574.
69. Rigotti G, Marchi A. Clinical treatment of radiotherapy damage. Plast Reconstr Surg. 2007;119(5):1409-1422. https://doi.org/10.1097/01.prs.0000256047.47909.71
70. Klinger M, Caviggioli F. Autologous fat graft in scar treatment. J Craniofac Surg. 2013;24(5):1610-1615. https://doi.org/10.1097/SCS.0b013e3182a24548
71. Cook JL, Chang AN. Fat grafting for chronic lower extremity wounds. Plast Reconstr Surg Glob Open. 2019;7(1):e2077.
72. Xiao S, Xiao C. Human fat products in wound healing meta-analysis. Int Wound J. 2024;21(2):e14463. https://doi.org/10.1111/iwj.70016
73. Marino G, Moraci M. Non-healing wounds treated with SVF. Regen Med. 2013;8(1):53-61.
74. Xiao S, Xiao C. Systematic review and meta-analysis. Int Wound J. 2024;21(2):e14463. https://doi.org/10.1111/iwj.70016
75. Gentile P, Garcovich S. ADSCs and SVF systematic review. Stem Cells Int. 2021;2021:6693709.
76. Cohen SR, Tijerina JD. Facial aging treatment with SVF. Plast Reconstr Surg. 2020;145(3):629e-642e.
77. Jiang A, Li M. SVF gel for facial rejuvenation. Stem Cell Res Ther. 2020;11(1):162. https://doi.org/10.1186/s13287-020-01811-7
78. Gontijo-de-Amorim NF, Silva GN. Mechanical SVF-enriched lipotransfer. Aesthet Surg J. 2017;37(8):868-877.
79. Cohen SR, Hewett S. Regenerative cells for facial surgery. Aesthet Surg J. 2017;37(Suppl 3):S16-S32. https://doi.org/10.1093/asj/sjx078
80. Simonacci F, Bertozzi N. Fat grafting complications. Ann Med Surg. 2017;20:49-60. https://doi.org/10.1016/j.amsu.2017.06.059
81. Largo RD, Tchang LA. Safety of fat grafting to breast. Breast. 2014;23(1):28-35.
82. Kato H, Mineda K. Fat grafting tissue remodeling. Plast Reconstr Surg. 2014;133(3):303e-313e. https://doi.org/10.1097/PRS.0000000000000066
83. Kim WS, Park BS. ADSC wound healing effects. J Dermatol Sci. 2007;48(1):15-24. https://doi.org/10.1016/j.jdermsci.2007.05.018
84. Khouri RK, Del Campo AF. Fat grafting surgical technique. Plast Reconstr Surg. 2009;124(1):272-280.
85. Coleman SR. Structural fat grafting principles. Plast Reconstr Surg. 2006;118(Suppl):108S-120S. https://doi.org/10.1097/01.prs.0000234610.81672.e7
86. Calabrese C, Garlaschi A. Oncological safety 5-year study. Plast Reconstr Surg. 2018;142(1):31-36.
87. Wang Y, Zhang J. Meta-analysis breast cancer patients. Aesthetic Plast Surg. 2023;47(2):451-462. https://doi.org/10.1007/s00266-023-03281-7
88. Petit JY, Botteri E. Fat grafting recurrence analysis. Plast Reconstr Surg. 2012;129(5):1173-1175.
89. Petit JY, Lohsiriwat V. Long-term oncological safety. Plast Reconstr Surg. 2011;128(3):643-653.
90. Kronowitz SJ, Mandujano CC. Breast cancer surveillance after grafting. Plast Reconstr Surg. 2016;137(4):1165-1175.
91. Teimourian B. Blindness following fat injections. Plast Reconstr Surg. 1988;82(2):361. https://doi.org/10.1097/00006534-198808000-00036
92. Dreizen NG, Framm L. Sudden unilateral visual loss after autologous fat injection. Am J Ophthalmol. 1989;107(1):85-87. https://doi.org/10.1016/0002-9394(89)90823-4
93. Danesh-Meyer HV, Savino PJ. Ocular and cerebral ischemia following facial injection. Arch Ophthalmol. 2001;119(5):777-778. https://doi.org/10.1001/archopht.119.5.777
94. Park SH, Sun HJ. Severe visual loss and cerebral infarction after autologous fat injection. Dermatol Surg. 2012;38(5):713-715.
95. Cervelli V, Gentile P. PRP and ADSCs for tissue regeneration. Regen Med. 2009;4(5):667-679.
96. Flynn L, Prestwich GD. Scaffolds for adipose engineering. Biomaterials. 2008;29(3):329-338. https://doi.org/10.1016/j.biomaterials.2007.12.028
97. Biancone L, Bruno S. MSC-derived microvesicles. Nephrol Dial Transplant. 2012;27(8):3037-3042. https://doi.org/10.1093/ndt/gfs168
98. Kim WS, Park BS. ADSC-derived exosomes. J Dermatol Sci. 2007;48(1):15-24.
99. Baptista LS, Silva KR. Depot-specific adipose stem cells. World J Stem Cells. 2015;7(1):165-173. https://doi.org/10.4252/wjsc.v7.i1.165
100. Goh BC, Thirumala S. Cryopreservation of adipose tissues. Cryobiology. 2007;55(1):1-9. https://doi.org/10.1002/term.35
101. US FDA. Minimal manipulation guidance. 2017.
102. Raposio E, Ciliberti R. European legislative issues. Ann Med Surg. 2017;24:61-64. https://doi.org/10.1016/j.amsu.2017.11.002
103. Aronowitz JA, Lockhart RA. Regulatory considerations. Springerplus. 2015;4:713. https://doi.org/10.1186/s40064-015-1509-2
104. Peltoniemi HH, Salmi A. Stem cell enrichment in fat grafting. Plast Reconstr Surg. 2013;131(4):701-711.
105. Kølle SF, Fischer-Nielsen A. ADSC enrichment clinical trial. Lancet. 2013;382(9898):1113-1120. https://doi.org/10.1016/S0140-6736(13)61410-5
106. Debuc B, Gendron N. Improving fat grafting CAL. Stem Cell Rev Rep. 2023;19(6):1726-1754. https://doi.org/10.1007/s12015-023-10568-4
107. Liu M, Shang Y. AFT retention strategies. Aesthetic Plast Surg. 2023;47(2):808-824. https://doi.org/10.1007/s00266-022-03088-y
108. Uguten UO, Veldhuizen J. Isolation systematic review. Plast Reconstr Surg Glob Open. 2024;12(1):e5534.
109.Jiang A, Li M. SVF gel applications. Stem Cell Res Ther. 2020;11(1):162. https://doi.org/10.1186/s13287-020-01811-7
110. Bora P, Majumdar AS. SVF in regenerative medicine. Stem Cell Res Ther. 2017;8(1):145. https://doi.org/10.1186/s13287-017-0598-y
111. Kølle SF, Fischer-Nielsen A. ADSC enrichment RCT. Lancet. 2013;382(9898):1113-1120. https://doi.org/10.1016/S0140-6736(13)61410-5
112. Casteilla L, Planat-Bénard V. Adipose stromal cells clinical. World J Stem Cells. 2011;3(4):25-33. https://doi.org/10.4252/wjsc.v3.i4.25
113. Zielins ER, Luan A. Therapeutic applications review. Discov Med. 2015;19(105):245-253.
2. Gause TM, Rubin JP. Adipose stem cells and fat grafting. Clin Plast Surg. 2014;41(2):345-352.
3. Debuc B, Gendron N, Cras A. Improving fat grafting through stem cell-assisted lipotransfer. Stem Cell Rev Rep. 2023;19(6):1726-1754. https://doi.org/10.1007/s12015-023-10568-4
4. Liu M, Shang Y, Liu N. Strategies to improve AFT retention. Aesthetic Plast Surg. 2023;47(2):808-824. https://doi.org/10.1007/s00266-022-03088-y
5. Chiu CH, Hsu CC, Lin YY. Cell-assisted lipotransfer in breast augmentation. Plast Reconstr Surg Glob Open. 2019;7(10):e2400.
6. Limido KE, Ma X. Long-term quantitative analysis of fat graft retention. J Plast Reconstr Aesthet Surg. 2023;78:44-52.
7. Cai Y, Zhang F, Feng J. Long-term follow-up of SVF gel in chronic wounds. Stem Cell Res Ther. 2024;15(1):85.
8. Gentile P, Garcovich S. Systematic review of ADSCs and SVF for wound healing. Stem Cells Int. 2021;2021:6693709.
9. Uguten UO, Veldhuizen J. Mechanical and enzymatic SVF isolation. Plast Reconstr Surg Glob Open. 2024;12(1):e5534.
10. Solodeev I, Marrazzo P. Comparison of enzymatic and mechanical methods. Aesthetic Plast Surg. 2023;47(3):1045-1054.
11. Kølle SF, Fischer-Nielsen A. Enrichment of fat grafts with ADSCs. Lancet. 2013;382(9898):1113-1120. https://doi.org/10.1016/S0140-6736(13)61410-5
12. Yoshimura K, Sato K. Cell-assisted lipotransfer for breast augmentation. Aesthetic Plast Surg. 2008;32(1):48-55. https://doi.org/10.1007/s00266-007-9019-4
13. Coleman SR. Facial recontouring with lipostructure. Clin Plast Surg. 1997;24(2):347-367. https://doi.org/10.1016/S0094-1298(20)31069-5
14. Simonacci F, Bertozzi N. Autologous fat grafting procedures. Ann Med Surg. 2017;20:49-60. https://doi.org/10.1016/j.amsu.2017.06.059
15. Eto H, Kato H. Fate of adipocytes after nonvascularized fat grafting. Plast Reconstr Surg. 2012;129(5):1081-1092. https://doi.org/10.1097/PRS.0b013e31824a2b19
16. Kato H, Mineda K. Degeneration and regeneration after fat grafting. Plast Reconstr Surg. 2014;133(3):303e-313e. https://doi.org/10.1097/PRS.0000000000000066
17. Bourin P, Bunnell BA. Stromal cells from adipose SVF. Cytotherapy. 2013;15(6):641-648. https://doi.org/10.1016/j.jcyt.2013.02.006
18. Yoshimura K, Shigeura T. Characterization of liposuction aspirate cells. J Cell Physiol. 2006;208(1):64-76. https://doi.org/10.1002/jcp.20636
19. Zuk PA, Zhu M. Multilineage cells from human adipose tissue. Tissue Eng. 2001;7(2):211-228. https://doi.org/10.1089/107632701300062859
20. Gimble JM, Katz AJ. Adipose-derived stem cells for regenerative medicine. Circ Res. 2007;100(9):1249-1260. https://doi.org/10.1161/01.RES.0000265074.83288.09
21. Matsumoto D, Sato K. Cell-assisted lipotransfer. Tissue Eng. 2006;12(12):3375-3382. https://doi.org/10.1089/ten.2006.12.3375
22. Matsumoto D, Gonda K. CAL-supportive use of ADSCs. J Cell Physiol. 2007;212(3):702-709.
23. Zielins ER, Luan A. Therapeutic applications of adipose stromal cells. Discov Med. 2015;19(105):245-253.
24. Bora P, Majumdar AS. Adipose SVF in regenerative medicine. Stem Cell Res Ther. 2017;8(1):145. https://doi.org/10.1186/s13287-017-0598-y
25. Zuk PA. The adipose-derived stem cell. Mol Biol Cell. 2010;21(11):1783-1787. https://doi.org/10.1091/mbc.e09-07-0589
26. Gimble JM, Bunnell BA. Adipose-derived stromal/stem cells primer. Organogenesis. 2013;9(1):3-10. https://doi.org/10.4161/org.24279
27. Dominici M, Le Blanc K. Minimal criteria for MSCs. Cytotherapy. 2006;8(4):315-317. https://doi.org/10.1080/14653240600855905
28. Mitchell JB, McIntosh K. Immunophenotype of adipose-derived cells. Stem Cells. 2006;24(2):376-385. https://doi.org/10.1634/stemcells.2005-0234
29. Miranville A, Heeschen C. Improvement of neovascularization by ADSCs. Circulation. 2004;110(3):349-355. https://doi.org/10.1161/01.CIR.0000135466.16823.D0
30. Planat-Benard V, Silvestre JS. Plasticity toward endothelial cells. Circulation. 2004;109(5):656-663. https://doi.org/10.1161/01.CIR.0000114522.38265.61
31. Corselli M, Chen CW. Perivascular ancestors of stem cells. Arterioscler Thromb Vasc Biol. 2010;30(6):1104-1109. https://doi.org/10.1161/ATVBAHA.109.191643
32. Tang W, Zeve D. White fat progenitor cells in vasculature. Science. 2008;322(5901):583-586. https://doi.org/10.1126/science.1156232
33. Spencer M, Yao-Borengasser A. Adipose tissue macrophages. Am J Physiol Endocrinol Metab. 2010;299(6):E1016-E1027. https://doi.org/10.1152/ajpendo.00329.2010
34. Shaul ME, Bennett G. M2-like remodeling phenotypes. Diabetes. 2010;59(5):1171-1181. https://doi.org/10.2337/db09-1402
35. Rehman J, Traktuev D. Secretion of angiogenic factors by adipose stromal cells. Circulation. 2004;109(10):1292-1298. https://doi.org/10.1161/01.CIR.0000121425.42966.F1
36. Kim WS, Park BS. Wound healing effect of ADSCs. J Dermatol Sci. 2007;48(1):15-24. https://doi.org/10.1016/j.jdermsci.2007.05.018
37. Cao Y, Sun Z. ADSCs differentiate into endothelial cells. Biochem Biophys Res Commun. 2005;332(2):370-379. https://doi.org/10.1016/j.bbrc.2005.04.135
38. Nakagami H, Maeda K. Autologous cell therapy in ischemic limb. Arterioscler Thromb Vasc Biol. 2005;25(12):2542-2547. https://doi.org/10.1161/01.ATV.0000190701.92007.6d
39. Rigotti G, Marchi A. Clinical treatment of radiotherapy damage. Plast Reconstr Surg. 2007;119(5):1409-1422. https://doi.org/10.1097/01.prs.0000256047.47909.71
40. Suga H, Eto H. Adipose tissue remodeling under ischemia. Plast Reconstr Surg. 2010;126(6):1911-1923. https://doi.org/10.1097/PRS.0b013e3181f4468b
41. Puissant B, Barreau C. Immunomodulatory effect of adipose stem cells. Br J Haematol. 2005;129(1):118-129. https://doi.org/10.1111/j.1365-2141.2005.05409.x
42. Yanez R, Lamana ML. ADSCs have immunosuppressive properties. Stem Cells. 2006;24(11):2582-2591. https://doi.org/10.1634/stemcells.2006-0228
43. Casteilla L, Planat-Bénard V. Adipose stromal cells in trials. World J Stem Cells. 2011;3(4):25-33. https://doi.org/10.4252/wjsc.v3.i4.25
44. Kilroy GE, Foster SJ. Cytokine profile of ADSCs. J Cell Physiol. 2007;212(3):702-709. https://doi.org/10.1002/jcp.21068
45. Tonnard P, Verpaele A. Nanofat grafting research and applications. Plast Reconstr Surg. 2013;132(4):1017-1026. https://doi.org/10.1097/PRS.0b013e31829fe1b0
46. Jiang A, Li M. Improvement via SVF gel grafting. Stem Cell Res Ther. 2020;11(1):162. https://doi.org/10.1186/s13287-020-01811-7
47. Cohen SR, Hewett S. Regenerative cells for facial surgery. Aesthet Surg J. 2017;37(Suppl 3):S16-S32. https://doi.org/10.1093/asj/sjx078
48. Flynn L, Prestwich GD. Adipose tissue engineering with scaffolds. Biomaterials. 2008;29(3):329-338. https://doi.org/10.1016/j.biomaterials.2007.12.028
49. Rodbell M. Metabolism of isolated fat cells. J Biol Chem. 1964;239:375-380. https://doi.org/10.1016/S0021-9258(18)51687-2
50. Bunnell BA, Flaat M. ADSC isolation and expansion. Methods. 2008;45(2):115-120. https://doi.org/10.1016/j.ymeth.2008.03.006
51. US FDA. Regulatory considerations for human cells and tissues. Guidance. 2017.
52. Raposio E, Ciliberti R. Clinical use of ADSCs: legislative issues. Ann Med Surg. 2017;24:61-64. https://doi.org/10.1016/j.amsu.2017.11.002
53. Aronowitz JA, Lockhart RA. Mechanical versus enzymatic isolation. Springerplus. 2015;4:713. https://doi.org/10.1186/s40064-015-1509-2
54. Tonnard P, Verpaele A. Nanofat grafting. Plast Reconstr Surg. 2013;132(4):1017-1026. https://doi.org/10.1097/PRS.0b013e31829fe1b0
55. Solodeev I, Marrazzo P. Comparison of isolation methods. Aesthetic Plast Surg. 2023;47(3):1045-1054.
56. Uguten UO, Veldhuizen J. Systematic review of yield and viability. Plast Reconstr Surg Glob Open. 2024;12(1):e5534.
57. Aronowitz JA, Ellenhorn JD. SVF isolation head-to-head comparison. Plast Reconstr Surg. 2013;132(4):932e-939e. https://doi.org/10.1097/PRS.0b013e3182a80652
58. Yoshimura K, Asano Y. Progenitor-enriched transplantation. Breast J. 2010;16(2):169-175. https://doi.org/10.1111/j.1524-4741.2009.00873.x
59. Khouri RK, Rigotti G. Megavolume autologous fat transfer. Plast Reconstr Surg. 2014;133(3):550-557. https://doi.org/10.1097/01.prs.0000438044.06387.2a
60. Chiu CH, Hsu CC. CAL in breast augmentation. Plast Reconstr Surg Glob Open. 2019;7(10):e2400.
61. Largo RD, Tchang LA. Efficacy and safety of fat grafting. Breast. 2014;23(1):28-35.
62. Calabrese C, Garlaschi A. CAL oncological safety study. Plast Reconstr Surg. 2018;142(1):31-36.
63. Wang Y, Zhang J. Fat grafting after breast cancer surgery meta-analysis. Aesthetic Plast Surg. 2023;47(2):451-462. https://doi.org/10.1007/s00266-023-03331-0
64. Petit JY, Botteri E. Locoregional recurrence after fat grafting. Plast Reconstr Surg. 2012;129(5):1173-1175.
65. Heine N, Sorg H. Fat grafting for breast reconstruction in radiated patients. J Plast Reconstr Aesthet Surg. 2023;76:234-242.
66. Salgarello M, Visconti G. Fat grafting before tissue expander in radiated patients. Plast Reconstr Surg. 2020;145(3):619e-628e.
67. Phothong W, Zenn MR. Fat grafting for radiation ulcer. Plast Reconstr Surg Glob Open. 2017;5(10):e1523.
68. Chen L, Li C. Autologous fat grafting after breast radiotherapy. Aesthetic Plast Surg. 2021;45(4):1565-1574.
69. Rigotti G, Marchi A. Clinical treatment of radiotherapy damage. Plast Reconstr Surg. 2007;119(5):1409-1422. https://doi.org/10.1097/01.prs.0000256047.47909.71
70. Klinger M, Caviggioli F. Autologous fat graft in scar treatment. J Craniofac Surg. 2013;24(5):1610-1615. https://doi.org/10.1097/SCS.0b013e3182a24548
71. Cook JL, Chang AN. Fat grafting for chronic lower extremity wounds. Plast Reconstr Surg Glob Open. 2019;7(1):e2077.
72. Xiao S, Xiao C. Human fat products in wound healing meta-analysis. Int Wound J. 2024;21(2):e14463. https://doi.org/10.1111/iwj.70016
73. Marino G, Moraci M. Non-healing wounds treated with SVF. Regen Med. 2013;8(1):53-61.
74. Xiao S, Xiao C. Systematic review and meta-analysis. Int Wound J. 2024;21(2):e14463. https://doi.org/10.1111/iwj.70016
75. Gentile P, Garcovich S. ADSCs and SVF systematic review. Stem Cells Int. 2021;2021:6693709.
76. Cohen SR, Tijerina JD. Facial aging treatment with SVF. Plast Reconstr Surg. 2020;145(3):629e-642e.
77. Jiang A, Li M. SVF gel for facial rejuvenation. Stem Cell Res Ther. 2020;11(1):162. https://doi.org/10.1186/s13287-020-01811-7
78. Gontijo-de-Amorim NF, Silva GN. Mechanical SVF-enriched lipotransfer. Aesthet Surg J. 2017;37(8):868-877.
79. Cohen SR, Hewett S. Regenerative cells for facial surgery. Aesthet Surg J. 2017;37(Suppl 3):S16-S32. https://doi.org/10.1093/asj/sjx078
80. Simonacci F, Bertozzi N. Fat grafting complications. Ann Med Surg. 2017;20:49-60. https://doi.org/10.1016/j.amsu.2017.06.059
81. Largo RD, Tchang LA. Safety of fat grafting to breast. Breast. 2014;23(1):28-35.
82. Kato H, Mineda K. Fat grafting tissue remodeling. Plast Reconstr Surg. 2014;133(3):303e-313e. https://doi.org/10.1097/PRS.0000000000000066
83. Kim WS, Park BS. ADSC wound healing effects. J Dermatol Sci. 2007;48(1):15-24. https://doi.org/10.1016/j.jdermsci.2007.05.018
84. Khouri RK, Del Campo AF. Fat grafting surgical technique. Plast Reconstr Surg. 2009;124(1):272-280.
85. Coleman SR. Structural fat grafting principles. Plast Reconstr Surg. 2006;118(Suppl):108S-120S. https://doi.org/10.1097/01.prs.0000234610.81672.e7
86. Calabrese C, Garlaschi A. Oncological safety 5-year study. Plast Reconstr Surg. 2018;142(1):31-36.
87. Wang Y, Zhang J. Meta-analysis breast cancer patients. Aesthetic Plast Surg. 2023;47(2):451-462. https://doi.org/10.1007/s00266-023-03281-7
88. Petit JY, Botteri E. Fat grafting recurrence analysis. Plast Reconstr Surg. 2012;129(5):1173-1175.
89. Petit JY, Lohsiriwat V. Long-term oncological safety. Plast Reconstr Surg. 2011;128(3):643-653.
90. Kronowitz SJ, Mandujano CC. Breast cancer surveillance after grafting. Plast Reconstr Surg. 2016;137(4):1165-1175.
91. Teimourian B. Blindness following fat injections. Plast Reconstr Surg. 1988;82(2):361. https://doi.org/10.1097/00006534-198808000-00036
92. Dreizen NG, Framm L. Sudden unilateral visual loss after autologous fat injection. Am J Ophthalmol. 1989;107(1):85-87. https://doi.org/10.1016/0002-9394(89)90823-4
93. Danesh-Meyer HV, Savino PJ. Ocular and cerebral ischemia following facial injection. Arch Ophthalmol. 2001;119(5):777-778. https://doi.org/10.1001/archopht.119.5.777
94. Park SH, Sun HJ. Severe visual loss and cerebral infarction after autologous fat injection. Dermatol Surg. 2012;38(5):713-715.
95. Cervelli V, Gentile P. PRP and ADSCs for tissue regeneration. Regen Med. 2009;4(5):667-679.
96. Flynn L, Prestwich GD. Scaffolds for adipose engineering. Biomaterials. 2008;29(3):329-338. https://doi.org/10.1016/j.biomaterials.2007.12.028
97. Biancone L, Bruno S. MSC-derived microvesicles. Nephrol Dial Transplant. 2012;27(8):3037-3042. https://doi.org/10.1093/ndt/gfs168
98. Kim WS, Park BS. ADSC-derived exosomes. J Dermatol Sci. 2007;48(1):15-24.
99. Baptista LS, Silva KR. Depot-specific adipose stem cells. World J Stem Cells. 2015;7(1):165-173. https://doi.org/10.4252/wjsc.v7.i1.165
100. Goh BC, Thirumala S. Cryopreservation of adipose tissues. Cryobiology. 2007;55(1):1-9. https://doi.org/10.1002/term.35
101. US FDA. Minimal manipulation guidance. 2017.
102. Raposio E, Ciliberti R. European legislative issues. Ann Med Surg. 2017;24:61-64. https://doi.org/10.1016/j.amsu.2017.11.002
103. Aronowitz JA, Lockhart RA. Regulatory considerations. Springerplus. 2015;4:713. https://doi.org/10.1186/s40064-015-1509-2
104. Peltoniemi HH, Salmi A. Stem cell enrichment in fat grafting. Plast Reconstr Surg. 2013;131(4):701-711.
105. Kølle SF, Fischer-Nielsen A. ADSC enrichment clinical trial. Lancet. 2013;382(9898):1113-1120. https://doi.org/10.1016/S0140-6736(13)61410-5
106. Debuc B, Gendron N. Improving fat grafting CAL. Stem Cell Rev Rep. 2023;19(6):1726-1754. https://doi.org/10.1007/s12015-023-10568-4
107. Liu M, Shang Y. AFT retention strategies. Aesthetic Plast Surg. 2023;47(2):808-824. https://doi.org/10.1007/s00266-022-03088-y
108. Uguten UO, Veldhuizen J. Isolation systematic review. Plast Reconstr Surg Glob Open. 2024;12(1):e5534.
109.Jiang A, Li M. SVF gel applications. Stem Cell Res Ther. 2020;11(1):162. https://doi.org/10.1186/s13287-020-01811-7
110. Bora P, Majumdar AS. SVF in regenerative medicine. Stem Cell Res Ther. 2017;8(1):145. https://doi.org/10.1186/s13287-017-0598-y
111. Kølle SF, Fischer-Nielsen A. ADSC enrichment RCT. Lancet. 2013;382(9898):1113-1120. https://doi.org/10.1016/S0140-6736(13)61410-5
112. Casteilla L, Planat-Bénard V. Adipose stromal cells clinical. World J Stem Cells. 2011;3(4):25-33. https://doi.org/10.4252/wjsc.v3.i4.25
113. Zielins ER, Luan A. Therapeutic applications review. Discov Med. 2015;19(105):245-253.
| Files | ||
| Issue | Vol 8 No 4 (2025) | |
| Section | Review Article(s) | |
| Keywords | ||
| Stromal vascular fraction adipose-derived stem cells fat grafting soft tissue reconstruction | ||
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
How to Cite
1.
Zamanitalab F. Autologous Fat Grafting Enhanced with Stromal Vascular Fraction (SVF) for Soft Tissue Reconstruction. AJS. 2025;8(4):151-156.
